mcb outlines

VIDEO PODCASTS & FLASH FILES ON MICROBIOLOGY LABORATORY TECHNIQUES

View the pre-lab files before doing the lab. Use the overview files as a review or guide to viewing the results of the lab. (These videos and the majority of the photographs were made in our lab of our materials. This is a work in progress - you can either download these (by right-clicking and selecting "save target as") to play on your computer or portable media player such as a Video Ipod, Zen Vision, or other brand - or you can play these files (by clicking on the link) on your computer. Let me know what you think!)

LAB	LAB OVERVIEW PODCAST(S)	PRE-LABS (FLASH FILES)
Lab Safety Features Overview	powerpoint showing the micro lab and its safety features
BASIC TECHNIQUES: Microscopy	Video showing the focusing of an oil immersion lens (wmv format)
MICROBIAL WORLD: Protozoans	powerpoint/video showing the non-parasitic protozoans	observe protozoans
MICROBIAL WORLD: Fungi	powerpoint/video showing the yeast & molds	observe representative fungi
MICROBIAL WORLD: Photosynthetic microbes		observe algae and cyanobacteria
MICROBIAL WORLD: Bacterial motility
BASIC TECHNIQUES: - loops and Bunsen burners	Video showing how to light a Bunsen burner Video - how to flame an inoculating loop (wmv format)	lighting and adjusting the Bunsen burner holding and flaming the loop
BASIC TECHNIQUES: transfering microbes		inoculate a tube and plate
BASIC TECHNIQUES: Isolation Streak Plate

STAINING TECHNIQUES: smears and the Simple Stain	powerpoint/video - how to do smears and the simple stain (wmv)	make a smear and simple stain
STAINING TECHNIQUES: Negative Stain		do the negative stain
STAINING TECHNIQUES: Capsule Stain		do the capsule stain (flash)
STAINING TECHNIQUES: Gram Stain	powerpoint/video showing how to do the Gram stain (wmv format)	do the Gram stain
STAINING TECHNIQUES: Acid Fast Stain, Spore Stain	powerpoint/video showing how to do the spore stain (wmv format)	do the acid fast stain
STAINING TECHNIQUES: Acid Fast Stain, Spore Stain	Video - how to heat the primary stain (wmv format)	do the spore stain

MEDIA: Blood Agar	powerpoint/video showing how to set up and read the blood plate (wmv)	set up the Blood agar
MEDIA: EMB Agar	powerpoint/video showing how to do the EMB plate (wmv format)	set up the EMB plate
MEDIA: Mannitol Salt Agar	powerpoint/video showing how to do the MSA plate (wmv format)	set up the MSA plate

GROWTH: Standard Plate Count	powerpoint/video how to do the dilutions and set up the plates (wmv)	set up the standard plate count
GROWTH: Standard Plate Count		counting the plates
GROWTH: Standard Plate Count
GROWTH: Media evaluation
GROWTH: Osmotic pressure
GROWTH: Aerotolerance

CONTROL: Antibiotic Sensitivity (Kirby-Bauer)		set up the antimicrobial sensitivity
CONTROL: Antibiotic Sensitivity	video showing how to place the discs on the Mueller-Hinton plate

GENETICS: DNA Fingerprinting	powerpoint/video showing how to set up and run the DNA gel (wmv format)
GENETICS: DNA Fingerprinting	Video showing how to load DNA samples in the agarose gel
GENETICS: DNA Fingerprinting	powerpoint/video showing how to read the results of the DNA gel

MICROBES & DISEASE: Protozoan Parasites	powerpoint/video showing the parasitic protozoans	look at the parasitic protozoans
MICROBES & DISEASE: Parasitic Worms	powerpoint/video showing the parasitic worms & vectors	look at parasitic worms
MICROBES & DISEASE: Vectors	powerpoint/video showing the parasitic worms & vectors	look at vectors

DIAGNOSTIC IMMUNOLOGY: Passive Agglutination	ppt/video showing the setup and reading of the passive agglutination	run the passive agglutination
DIAGNOSTIC IMMUNOLOGY: Passive Agglutination	video showing how to spread the reagents on the slide (mpeg format)
DIAGNOSTIC IMMUNOLOGY: Passive Agglutination	video showing how to rotate the slide to view the reaction (mpeg)
DIAGNOSTIC IMMUNOLOGY: Immunoprecipitation	powerpoint showing the setup and reading of the immunoprecipitation plate	set up the immunoprecipitation

IDENTIFYING MICROBES: Enterotube	ppt/video showing the setup and reading of the enterotube (m4v format)	inoculate the Enterotube (flash)
QUANTITATION: Soil Microbial Count		set up the soil count plates

BIOCHEMICAL TESTING: Sugar fermentation	powerpoint showing the setup of the sugar fermentation tubes and how to read the results	set up the sugar fermenation phenol red broths
BIOCHEMICAL TESTING: IMViC Tests (Indole, MRVP, Citrate)	powerpoint showing how to inoculate and read all of these tests	inoculate the IMViC media
BIOCHEMICAL TESTING: TSI Agar	powerpoint showing how to set up and read the TSI agar	inoculate the TSI agar
BIOCHEMICAL TESTING: Litmus Milk	powerpoint showing how to set up and read the litmus milk reactions

BIOCHEMICAL TESTING: DNase		read the DNase plate
BIOCHEMICAL TESTING: Gelatin hydrolysis	powerpoint/video showing how to inoculate the gelatin tubes and how to read the results (m4v)	set up the gelatinase tubes
BIOCHEMICAL TESTING: Starch Hydrolysis		set up the starch plate read the starch plate
BIOCHEMICAL TESTING: Casien Hydrolysis / Casease	powerpoint showing the setup and reading of the milk / casien plates	set up the casease (milk) plate
BIOCHEMICAL TESTING: Urease Test	powerpoint/video showing setup and reading of the urease tubes (m4v)	inoculate the urease broth
BIOCHEMICAL TESTING: Nitrate Reduction Test

OXYGEN METABOLISM: Catalase test		do the catalase test (flash)
OXYGEN METABOLISM: Oxidase Test	powerpoint showing how to do and read the results of the oxidase test